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WASEDA'S REGIMEN |
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WASEDA'S COMPREHENSIVE
ANALYSIS ON HAIR LOSS
(6) Br J Dermatol 2000 Nov;143(5):1036-1039 High-dose proinflammatory cytokines induce apoptosis of hair bulb keratinocytes in vivo. Ruckert R, Lindner G, Bulfone-Paus S, Paus R Institute of Immunology, Benjamin Franklin Medical Center, Free University Berlin, Berlin, Germany; Department of Dermatology, Virchow Hospital/Charite, Berlin, Germany; Department of Dermatology, University Hospital Eppendorf, University of Hamburg, Martinistr. 52, D-20246 Hamburg, Germany. BACKGROUND: Hair loss
following skin inflammation may in part be mediated by keratinocyte (KC)
apoptosis. While the effects of different cytokines or other apoptosis
stimulating agents such as interferon (IFN)-gamma or tumour necrosis
factor (TNF)-alpha on KC apoptosis in vitro have been addressed in several
studies, little is known about the effects of proinflammatory cytokines on
KC apoptosis in vivo. Objectives To study the effects of intradermally
injected TNF-alpha, interleukin (IL)-1beta and IFN-gamma on KC apoptosis
in the back skin of C57BL/6 mice. METHODS: Apoptosis in epidermal and hair
bulb KCs was analysed by immunohistology using TUNEL staining. RESULTS:
Injection of TNF-alpha induced a significantly higher number of apoptotic
cells within the epidermis than vehicle; all three proinflammatory
cytokines together further increased their number. Intrafollicular hair
bulb KCs were much more susceptible to apoptosis induction by TNF-alpha or
IL-1beta; their injection significantly upregulated apoptosis after 6 h,
which was further increased after 24 h. The combination of all cytokines
together accelerated intrafollicular apoptosis after 6 h by doubling the
number of apoptotic cells per hair bulb, compared with the effects of TNF-alpha
or IL-1beta alone. PMID: 11069516
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