WASEDA'S REGIMEN
AMASHISO + ANALYSIS ON HAIR LOSS

WASEDA'S COMPREHENSIVE ANALYSIS ON HAIR LOSS 
&
INTRODUCTION TO AMASHISO
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the following is reprinted from a series of posts submitted by Waseda in forum # 22.

(4) J Agric Food Chem 1999 Feb;47(2):468-72

Glycoprotein derived from the hot water extract of mint plant, Perilla frutescens britton.

Asada M, Fukumori Y, Inoue M, Nakagomi K, Sugie M, Fujita Y, Tomizuka N, Yamazaki Y, Oka S

Cell Biology Laboratory, Biosignalling Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan.

Glycoprotein showing inhibitory activity against mast cell degranulation and hyaluronidase activity was purified from the hot water extract of mint plant (Perilla frutescens Britton). The purified inhibitor gave a single band detected with Coomassie brilliant blue staining and periodic acid-Schiff staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The molecular mass was estimated to be 6.0 kDa on SDS-PAGE. The inhibitor did not become inactivated when boiled for 30 min or digested with trypsin, V8 protease, or proteinase K but was inactivated by NaIO(4) oxidation. The inhibitor prevented mast cell degranulation and hyaluronidase activity (IC(50) = 0.42 mg/mL) in a dose-dependent manner. The inhibitor also inhibited the protein kinase C activity. It is possible to purify and characterize a glycoprotein with putative pharmacological properties from mint plants.

PMID: 10563918, UI: 20031801

 
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Footnotes to Waseda's Analysis

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